Reversed Phase Columns 1

HYDROCELL RP 5S and RP 10S are produced from new particles with particle size 5 µm and 10 µm (d₅₀) respectively.The pore size are about 500Å respectively. These particles have high pore volume and large pore size. The major advantages of these new media are lower back pressure and higher permeability in the operation. The media reduce the fluctuation and drop of back pressure when it was used in the gradient separation.

• RP 5S is prepared from 5 µm (d₅₀), average 500 Å spherical PS-DVB particles. The media provide optimum pore volume and pore size with increasing permeability and high separating efficiency for small drugs, peptides and other small biomolecules.
• RP 10S is prepared from 10 µm (d₅₀), average 500 Å spherical PS-DVD particles which give increased permeability, reducing the fluctuation and drop of back pressure and is suitable for the separation of peptides, oligonucleotide and other biomolecules.

Hydrocell RP 10S
Antibiotic Mixture		Peptide Standard
Column:50 x 4.6 mm Mobile Phase: A: 5% Acetonitrile in 0.1% TFA B:95% Acetonitrile in 0.1% TFA		Column:150 x 4.6 mm Mobile Phase: A: 5% Acetonitrile in 0.1% TFA B:95% Acetonitrile in 0.1% TFA
Gradient: Linear, 5-30% B in 30 mins Flow Rate:1.0 mL/min Detection:UV, 280 nm Peak Identification: 1. Cefotaxime 2. Cefazolin		Gradient: Linear, 5-35% B in 40 mins Flow Rate: 1.0 mL/min Detection: UV, 280 nm Peak Identification: 1. Gly-Tyr 2. Val-Tyr-Val 3. Methionine Enkephalin 4. Leucine Enkephalin 5. Angiotensin II

Hydrocell RP 5S
Antibiotic Mixture		Peptide Standard
Column:50 x 4.6 mm Mobile Phase: A: 5% Acetonitrile in 0.1% TFA B:95% Acetonitrile in 0.1% TFA		Column:150 x 4.6 mm Mobile Phase: A: 5% Acetonitrile in 0.1% TFA and 10 mM Tris-HCl B:80% Acetonitrile in 0.1% TFA and 10 mM Tris-HCl
Gradient: Linear, 5-30% B in 30 mins Flow Rate:1.0 mL/min Detection:UV, 280 nm Peak Identification: 1. Cefotaxime 2. Cefazolin		Gradient: Linear, 5-35% B in 40 mins Flow Rate: 1.0 mL/min Detection: UV, 280 nm Peak Identification: 1. Gly-Tyr 2. Val-Tyr-Val 3. Methionine Enkephalin 4. Leucine Enkephalin 5. Angiotensin II

Comparison of Hydrocell RP 5S and Waters Symmetry C18 Column
in the Separation of Pharmaceutical Polypeptide Product and Substrate.

BioChrom RP 5S		Waters Symmetry C18
Column : 50 x 4.6 mm		Column:75 x4.6 mm
Sample: Polypeptides		Sample: Polypeptides
Particle Size: 5 µm		Particle Size: 3.5 µm
pH Range :1-14		pH Range: 3-8
N=10,450		N=7,452
Mobile Phase A: 20/72/8 /0.1% 50mM Tris/ACN/water/TFA Mobile PhaseB: 20/8/72/0.1% 50mM Tris/ACN/water/TFA Flow Rate: 1 mL/min. Gradient: 20% to 70 % A in 4 mins. 70 to 100% A in 0.5 mins. 100% A for 1.5 mins.		Mobile Phase A: 90/10 /0.1% ACH/water/TFA Mobile PhaseB: 10/90/0.1% ACN/water/TFA Flow Rate: 1 mL/min. Gradient: 20%A hold 2 mins. 20 to 80 % A in 3 mins.