|
|
|
|
Reversed Phase Columns
|
|
HYDROCELL RP 5S and RP 10S are
produced from PS-DVB particles with particle size 5 µm and 10 µm (d50)
respectively. The pore size are about 500Å
respectively. These particles have high pore volume and large pore size.
The major advantages of these new media are lower back pressure and higher
permeability in the operation. The media reduce the fluctuation and drop of
back pressure when it was used in the gradient separation.
- RP
5S
is
prepared from 5 µm (d50), average 500 Å spherical PS-DVB
particles. The media provide optimum pore volume and pore size with increasing permeability and high separating
efficiency for small drugs, peptides and other small biomolecules.
- RP
10S
is prepared from 10 µm (d50), average
500 Å spherical PS-DVD particles which give increased permeability,
reducing the fluctuation and drop of back pressure and is suitable for
the separation of peptides, oligonucleotide
and other biomolecules.
 Chromatogram
of Antibiotics on RP 5S Column 50x4.6 mm
Chromatogram
of Peptides on RP 5S Column 50x4.6 mm
Chromatogram
of Antibiotics on RP 10S Column 50x4.6 mm
Chromatogram
of Peptides on RP 10S Column 50x4.6 mm
|
|
|
|
Comparison
of Hydrocell RP 5S and Waters Symmetry C18 Column
in the Separation of Pharmaceutical Polypeptide Product
and Substrate.
|
|
|
|
RP
5G and RP 10G are produced from highly cross-linked
Polystyrene-divinyl benzene (PS-DVB) spherical
beads. The surface of the materials has been modified by hydrophilic
coating to reduce the hydrophobicity and the
small pores of these particles have been sealed to prevent the trapping of biomolecules. These supports are suitable for reversed
phase analysis and purification of small polypeptides, oligonucleotides
and proteins.
- RP
5G
is
prepared from 5 µm, 500 Å spherical PS-DVB particles with hydrophilic
surface coating. This polymer provides optimum surface area with high
separating efficiencies for small drug, and polypeptides.
- RP
10G
is
prepared from 10 µm, 500 Å spherical PS-DVB particles with hydrophilic
surface coating. This polymer provides optimum surface area with high
separating efficiencies for polypeptides, proteins and oligonucleotides.
These materials are
extremely stable and permit use of mobile phase pH from 1 to 14 and the
maximum temperature limit is up to 80° c. Reversed phase chromatography is
based on the discrimination by hydrophobicity of biomolecules and is a complementary technique to ion
exchange and hydrophobic interaction chromatography.
Chromatogram
of peptides on RP 5G Column
Chromatogram
of Antibiotics on RP 5G Column
Chromatogram
of Proteins on RP 10G Column
|
|
|
HYDROCELL RP 5D and RP 10D are produced from macroporous PS-DVB beads with particle size 5 µm and 10
µm (d50) respectively. These particles have high pore volume and
permeability. Their major advantages are lower back pressure and higher
permeability in the operation. These media reduce the fluctuation and drop of
back pressure when it was used in the gradient separations.
- RP 5D
and 10D is prepared from 5 µm and 10
µm (d50), 1500 Å spherical PS-DVB particles. The media
provide optimum pore volume and large pore size with increasing
permeability and high separating efficiency for large proteins,
polypeptides, oligonucleotides and other
macromolecules.
Chromatogram
of protein mixture on RP 5D Column 150 x 2.1 mm
Chromatogram
of Protein Mixture on RP 10D Column 150 x 4.6 mm
Chromatogram
of Protein Mixture on RP 10D Column 150 x 2.1 mm
Hydrocell Reversed Phase Columns RP
10D and RP 5D for Direct High Molecular Weight of Protein and Enzyme Analyses
and Purification
BioChrom Labs, Inc.
provides innovative macroporous
polymer beads (RP 10D and 5D) for direct protein and enzyme analysis and
purification. Hydrocell Reversed Phase Columns are produced from high
cross-linked, macroporous PS-DVB polymer beads.
They can be used to analyze and separate small peptide, polypeptides and high
molecular weight proteins and enzymes in a single column. The Hydrocell
Reversed Phase columns effectively separate and analyze molecular weights of biomolecules more than 290 KDa in size. It is easy during
the method development to use Hydrocell Reversed Phase columns for
biomolecular separation and analysis. The gradient profiles of RP 10D
and RP 5D are similar to gradient profiles in the conventional Silica based
C-18 columns. Under the what is new section of the BioChrom Website, you can see the demonstrating
chromatograms by using the RP 5D column to separate small peptides 2-10 amino
acids, and analyze commercial human insulin and bovine
insulin. You can also see the demonstrating chromatograms by
using the RP 10D column to separate standard proteins molecular weight from
12.3 KDa. to 44.3 KDa., to separate high molecular
weight of proteins and enzymes from 12.3 KDa. to 290
KDa., and to purify thyroglobulin, molecular weight
670 KDa.
Chromatogram of small peptides on RP 5D
Column, 150 x 4.6 mm
Chromatogram of
standard proteins on RP 5D Column, 150 x 4.6 mm
Chromatogram of
commercial human insulin on RP 5D Column, 150 x 2.1 mm
Chromatogram of
Bovine pancreas insulin on RP 5D Column, 150 x 2.1 mm
Chromatogram of
standard proteins on RP 10D Column, 150 x 4.6 mm
Chromatogram
of albumin on RP 10D Column, 150 x 2.1 mm
Chromatogram
of high M.W. proteins & enzymes on RP 10D Column, 150 x 2.1 mm
Chromatogram
of Thyroglobulin on RP 10D Column, 150 x 2.1 mm
|
|
|
