BioChrom Labs, Inc.

BioChrom Labs, Inc.

BioChrom Labs, Inc.

BioChrom Labs, Inc.

 

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HYDROCELL HPLC Column

 

 


Reversed Phase Columns



HYDROCELL RP 5S and RP 10S are produced from PS-DVB particles with particle size 5 m and 10 m (d50) respectively. The pore size are about 500 respectively. These particles have high pore volume and large pore size. The major advantages of these new media are lower back pressure and higher permeability in the operation. The media reduce the fluctuation and drop of back pressure when it was used in the gradient separation.

  • RP 5S is prepared from 5 m (d50), average 500 spherical PS-DVB particles. The media provide optimum pore volume and pore size with increasing permeability and high separating efficiency for small drugs, peptides and other small biomolecules.
  • RP 10S is prepared from 10 m (d50), average 500 spherical PS-DVD particles which give increased permeability, reducing the fluctuation and drop of back pressure and is suitable for the separation of peptides, oligonucleotide and other biomolecules.

ViewChromatogram of Antibiotics on RP 5S Column 50x4.6 mm

ViewChromatogram of Peptides on RP 5S Column 50x4.6 mm

ViewChromatogram of Antibiotics on RP 10S Column 50x4.6 mm

ViewChromatogram of Peptides on RP 10S Column 50x4.6 mm

 


ViewComparison of Hydrocell RP 5S and Waters Symmetry C18 Column
             
in the Separation of Pharmaceutical Polypeptide Product and Substrate.

 


RP 5G and RP 10G are produced from highly cross-linked Polystyrene-divinyl benzene (PS-DVB) spherical beads. The surface of the materials has been modified by hydrophilic coating to reduce the hydrophobicity and the small pores of these particles have been sealed to prevent the trapping of biomolecules. These supports are suitable for reversed phase analysis and purification of small polypeptides, oligonucleotides and proteins.

  • RP 5G is prepared from 5 m, 500 spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiencies for small drug, and polypeptides.
  • RP 10G is prepared from 10 m, 500 spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiencies for polypeptides, proteins and oligonucleotides.

These materials are extremely stable and permit use of mobile phase pH from 1 to 14 and the maximum temperature limit is up to 80 c. Reversed phase chromatography is based on the discrimination by hydrophobicity of biomolecules and is a complementary technique to ion exchange and hydrophobic interaction chromatography.

ViewChromatogram of peptides on RP 5G Column

ViewChromatogram of Antibiotics on RP 5G Column

ViewChromatogram of Proteins on RP 10G Column

 


HYDROCELL RP 5D and RP 10D are produced from macroporous PS-DVB beads with particle size 5 m and 10 m (d50) respectively. These particles have high pore volume and permeability. Their major advantages are lower back pressure and higher permeability in the operation. These media reduce the fluctuation and drop of back pressure when it was used in the gradient separations.

  • RP 5D and 10D is prepared from 5 m and 10 m (d50), 1500 spherical PS-DVB particles. The media provide optimum pore volume and large pore size with increasing permeability and high separating efficiency for large proteins, polypeptides, oligonucleotides and other macromolecules.

ViewChromatogram of protein mixture on RP 5D Column 150 x 2.1 mm

ViewChromatogram of Protein Mixture on RP 10D Column 150 x 4.6 mm

ViewChromatogram of Protein Mixture on RP 10D Column 150 x 2.1 mm

Hydrocell Reversed Phase Columns RP 10D and RP 5D for Direct High Molecular Weight of Protein and Enzyme Analyses and Purification

 

BioChrom Labs, Inc. provides innovative macroporous polymer beads (RP 10D and 5D) for direct protein and enzyme analysis and purification. Hydrocell Reversed Phase Columns are produced from high cross-linked, macroporous PS-DVB polymer beads. They can be used to analyze and separate small peptide, polypeptides and high molecular weight proteins and enzymes in a single column. The Hydrocell Reversed Phase columns effectively separate and analyze molecular weights of biomolecules more than 290 KDa in size. It is easy during the method development to use Hydrocell Reversed Phase columns for biomolecular separation and analysis.  The gradient profiles of RP 10D and RP 5D are similar to gradient profiles in the conventional Silica based C-18 columns. Under the what is new section of the BioChrom Website,  you can see the demonstrating chromatograms by using the RP 5D column to separate small peptides 2-10 amino acids, and analyze commercial human insulin and bovine insulin.   You can also see the demonstrating chromatograms by using the RP 10D column to separate standard proteins molecular weight from 12.3 KDa. to 44.3 KDa.,  to separate high molecular weight of proteins and enzymes from 12.3 KDa. to 290 KDa., and to purify thyroglobulin, molecular weight 670 KDa. 


ViewChromatogram of small peptides on RP 5D Column, 150 x 4.6 mm

ViewChromatogram of standard proteins  on RP 5D Column, 150 x 4.6 mm

ViewChromatogram of commercial human insulin  on RP 5D Column, 150 x 2.1 mm

ViewChromatogram of Bovine pancreas insulin on RP 5D Column, 150 x 2.1 mm

ViewChromatogram of standard proteins  on RP 10D Column, 150 x 4.6 mm

ViewChromatogram of albumin on RP 10D Column, 150 x 2.1 mm

ViewChromatogram of high M.W. proteins & enzymes  on RP 10D Column, 150 x 2.1 mm

  ViewChromatogram of Thyroglobulin  on RP 10D Column, 150 x 2.1 mm

 

 

 

 

 

 

 

 

 

 

 


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