BioChrom Labs, Inc. - HYDROCELL HPLC Column

Reversed Phase HPLC Columns

Reversed Phase Columns

HYDROCELL RP 5S and RP 10S are produced from PS-DVB particles with particle size 5 µm and 10 µm (d₅₀) respectively. The pore size are about 500Å respectively. These particles have high pore volume and large pore size. The major advantages of these new media are lower back pressure and higher permeability in the operation. The media reduce the fluctuation and drop of back pressure when it was used in the gradient separation.

RP 5S is prepared from 5 µm (d₅₀), average 500 Å spherical PS-DVB particles. The media provide optimum pore volume and pore size with increasing permeability and high separating efficiency for small drugs, peptides and other small biomolecules.
RP 10S is prepared from 10 µm (d₅₀), average 500 Å spherical PS-DVD particles which give increased permeability, reducing the fluctuation and drop of back pressure and is suitable for the separation of peptides, oligonucleotide and other biomolecules.

Chromatogram of Antibiotics on RP 5S Column 50x4.6 mm

Chromatogram of Peptides on RP 5S Column 50x4.6 mm

Chromatogram of Antibiotics on RP 10S Column 50x4.6 mm

Chromatogram of Peptides on RP 10S Column 50x4.6 mm

Comparison of Hydrocell RP 5S and Waters Symmetry C18 Column
in the Separation of Pharmaceutical Polypeptide Product and Substrate.

RP 5G and RP 10G are produced from highly cross-linked Polystyrene-divinyl benzene (PS-DVB) spherical beads. The surface of the materials has been modified by hydrophilic coating to reduce the hydrophobicity and the small pores of these particles have been sealed to prevent the trapping of biomolecules. These supports are suitable for reversed phase analysis and purification of small polypeptides, oligonucleotides and proteins.

RP 5G is prepared from 5 µm, 500 Å spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiencies for small drug, and polypeptides.
RP 10G is prepared from 10 µm, 500 Å spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiencies for polypeptides, proteins and oligonucleotides.

These materials are extremely stable and permit use of mobile phase pH from 1 to 14 and the maximum temperature limit is up to 80° c. Reversed phase chromatography is based on the discrimination by hydrophobicity of biomolecules and is a complementary technique to ion exchange and hydrophobic interaction chromatography.

Chromatogram of peptides on RP 5G Column

Chromatogram of Antibiotics on RP 5G Column

Chromatogram of Proteins on RP 10G Column

HYDROCELL RP 5D and RP 10D are produced from macroporous PS-DVB beads with particle size 5 µm and 10 µm (d₅₀) respectively. These particles have high pore volume and permeability. Their major advantages are lower back pressure and higher permeability in the operation. These media reduce the fluctuation and drop of back pressure when it was used in the gradient separations.

RP 5D and 10D is prepared from 5 µm and 10 µm (d₅₀), 1500 Å spherical PS-DVB particles. The media provide optimum pore volume and large pore size with increasing permeability and high separating efficiency for large proteins, polypeptides, oligonucleotides and other macromolecules.

Chromatogram of protein mixture on RP 5D Column 150 x 2.1 mm

Chromatogram of Protein Mixture on RP 10D Column 150 x 4.6 mm

Chromatogram of Protein Mixture on RP 10D Column 150 x 2.1 mm

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