BioChrom RP 5S Waters Symmetry C18
Column : 50 x 4.6 mm Column:75 x4.6 mm
Sample: Polypeptides Sample: Polypeptides
Particle Size: 5 µm Particle Size: 3.5 µm
pH Range :1-14 pH Range: 3-8
N=10,450 N=7,452
   
Mobile Phase A: 20/72/8 /0.1%
50mM Tris/ACN/water/TFA

Mobile PhaseB:20/8/72/0.1%
50mM Tris/ACN/water/TFA

Flow Rate: 1 mL/min.

Gradient: 20% to 70 % A in 4 mins. 70 to 100% A in 0.5 mins. 100% A for 1.5 mins.

 Mobile Phase A:90/10 /0.1%
ACH/water/TFA

Mobile PhaseB:10/90/0.1%
ACN/water/TFA

Flow Rate: 1 mL/min.

Gradient: 20%A hold 2 mins. 20 to 80 % A in 3 mins.