Hydrocell RP 10D
Protein Mixture
Column: 150 x 2.1 mm
A: 5% Acetonitrile in 0.1% TFA
B:95% Acetonitrile in 0.1% TFA
Gradient:
Linear, 20-70% B in 25 mins
Flow Rate:0.25 mL / min
Detection:
UV, 280 nm
Peak Identification:
1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
4. Myoglobin
5. Ovalbumin
