Hydrocell RP 5D

Protein Mixture

Column: 150 x 2.1 mm
Mobile Phase:
A: 5% Acetonitrile in 0.1% TFA
B:95% Acetonitrile in 0.1% TFA

Gradient:
Linear, 20-70% B in 25 mins

Flow Rate:0.25 mL / min

Detection:UV, 280 nm

Peak Identification:
1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
4. Myoglobin
5. Ovalbumin