Mobile Phase:
Eluent A: 25 mM CHES, pH 8.0
Eluent B: Eluent A + 1.2 M Ammonium Sulfate, pH 8.0

Gradient: Linear 10-50% B in 40 minutes

Flow Rate: 0.25 mL / min.

Detection: UV 260 nm

Samples: OLigothymidylic Acid d( pT) 12-18 mer, 5 units in 1 mL of eluent A

Injection 5 µL