DEAE NP10

Column: 35x4.6 mm

Mobile Phase:
A: 2 M Urea, 10 mM Potassium Phosphate, pH 5.5
B: Eluent A + 0.5 M Ammonium Sulfate, pH 5.5

Gradient:
Linear 40-100% B in 30 minutes

Flow Rate: 1 mL/min

Detection: UV 280 nm

Samples:
pBR322 DNA Hae III Digest