The HYDROCELL NP10 media do not readily change their physical dimensions under high flow rate or under steep gradient applications. The analysis of proteins and enzymes can be conducted with short run times by either increasing the gradient slope or the flow rate.

However, the flow rate does not contribute to the shortness of the analysis time as much as the gradient slope does, especially for high retention macromolecules.

The high speed, high resolution separation can be achieved at a moderate flow rate with high gradient slope on a HYDROCELL NP10 column to reduce the solvent consumption.

As shown in the following chromatograms, an increase in flow rate at the same gradient time (a & b, or c & d) sharpens peaks and slightly decreases run time, while a decrease in gradient time at the same flow rate (a & c or b & d) dramatically reduces run time.

Chromatogram of protein mixture on C3 NP10