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Protein Mixture
Column:
4.6 x 35 mm
Mobile
Phase:
A: 20 mM
Potassium Phosphate, pH 6.0
B: Eluent A + 0.5 M NaCl, pH 6.0
Gradient:
Linear 0-100% B in 5 minutes
Detection:
UV 280 nm
Flow Rate:
2.0 mL/min
Injection:
10 µL
Peak
Identification:
1.
Myoglobin
2. Trysinogen
3. Chymotrysinogen A
4. Lysozyme
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