|
Protein Mixture
Column:
35 x 4.6 mm
Mobile
Phase:
A: 10 mM Tris, pH 8.0
B: Eluent A + 0.5 M NaCl, pH 8.0
Gradient:
Linear 0-50% B in 5 minutes
Detection:
UV 280 nm
Flow Rate:
2 mL / min
Injection:
10 µL
Peak
Identification:
1.
Myoglobin
2. Conalbumin
3. Ovalbumin
4. Soybean Trypsin Inhibitor |