Hydrocell Phenyl NP10

Column: 150 x4.6 mm
Mobile Phase:
Eluent A: 2.5 M Ammonium Sulfate in Eluent B
Eluent B: 0.1 M Potassium Phosphate (pH 7.0)
Gradient: Linear 0-100% B in 20 minutes
Detection: UV 280 nm
Flow Rate: 1.0 mL / min.

Peak Identification:
1. Ribonuclease A
2. Lysozyme
3. Chymotrysinogen A