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Protein Mixture
Column: 35 x 4.6 mm
Mobile Phase:
A: 10 mM Tris, pH 8.0
B: Eluent A + 0.5 M NaCl, pH8.0
Gradient: Linear 0-50% B in 5 minutes
Detection: UV 280 nm
Flow Rate: 2.0 mL / min.
Injection: 10 µL
Peak Identification:
1. Myoglobin
2. Conalbumin
3. Ovalbumin
4. Soybean Trypsin Inhibitor
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