Hydrocell CM NP10

Column: 4.6 x 35 mm

Mobile Phase:
Eluent A: 20 mM Potassium Phosphate, pH 6.0
Eluent B: Eluent A + 0.5 M NaCl, pH 6.0
Gradient: Linear 0-100% B in 5 minutes
Detection: UV 280 nm
Flow Rate: 2 mL/min
Injection: 20 µL

Peak Identification:
1. Trysinogen
2. Chymotrysinogen A
3. Lysozyme