Hydrocell C4 NP10

Protein Mixture

Column: 35 x4.6 mm

Mobile Phase:
A: 2.5 M Ammonium Sulfate in Eluent B
B: 0.1 M Potassium Phosphate (pH 7.0)
Gradient: Linear 0-100% B in 5 minutes
Detection: UV 280 nm
Flow Rate: 2.0 mL/min
Injection: 10 µL

Peak Identification:
     1. Cytochrome C
     2. Ribonuclease A
     3. Lysozyme
     4. Chymotrysinogen A