Hydrocell C3 NP10

Protein Mixture

Column: 50 x4.6 mm

Mobile Phase:
A: 2.5 M Ammonium Sulfate in Eluent B
B: 0.1 M Potassium Phosphate (pH 7.0)
Gradient: Linear 0-100% B in 20 minutes
Detection: UV 280 nm
Flow Rate: 1.0 mL/min
Injection: 20 µL

Peak Identification:
     1. Ribonuclease A
     2. Lysozyme
     3. Chymotrysinogen A