Ligand Exchange HPLC Column

        Ligand exchange columns are use for the analysis of carbohydrates, alcohols and organic acids. However, most columns
 in the market are produced from low crosslinked resin and limited the speed of separations because of back pressure. BioChrom
developed ligand exchange HPLC column, Hydrocell SCX 100 by sulfonating the high crosslinked polystyrene-divinylbenzene
beads with average particle size 5 µm and  pore size 100 Å.

The resin can be changed its selectivity by ligand exchange interaction with metal ion such as  sodium, calcium and lead. The selection
 of molecular weight range of analysis can also be controlled by changing  crosslinked percentage of resin. Pharmacopeia methods
specify the type of HPLC column which should be used. Hydrocell SCX 100 column comply with USP definitions of Media Type L17.
The ligand exchange interaction with calcium and lead of  H-form resin can change its application to comply with definitions of  media
type L19 and L34.

The typical operating conditions of the column is eluted under isocratic condition with water which has been adjusted to pH  2.5
by sulfuric acid solution at flow rate 1.5 mL/minute  in 7.8 x 100 mm dimension of column at room temperature. The recommendation
of maximum flow rate of this column is 6 mL / minutes. It is recommended  to clean this column with 0.5 M sulfuric acid, 0.5 M sodium
 hydroxide or 80% Methanol.

The separation conditions of this column to monitor the purity of  small drug, Ribavirin are as follows.
 

Hydrocell SCX 100
Column: 7.8 x 100 mm
Mobile Phase: Water , pH 2.5 (sulfuric Acid) Elution: Isocratic Detection: UV 207 nm Flow Rate: 1.5 mL/minute (back pressure 600psi) Sample: 6 ppm Ribavirin Injection: 10 µL Catalog #: 43-21SCX