Weak Cation Exchanger: CM 1500 and CM 3000

CM 1500 is prepared from 10 µm particles of highly cross-linked PS-DVB beads with pore diameter of 500 Å. Similarly, CM 3000 is prepared from 10 µm particles of highly cross-linked PS-DVB beads with pore diameter of larger than 1000 Å. The polymer has been completely covered with neutral, hydrophilic layer to shield the hydrophobic surface.

In coupling of the polymer chains of carboxymethyl (CM) groups with the rigid macroporous polymer matrix, weak cation separations can be achieved over the wide pH range of 5 to 12.

The separation is usually accomplished with salts or pH gradients using an aqueous mobile phase. The dynamic loading capacity for lysozyme is ca. 45 mg/mL of column volume. Recoveries of proteins are typically from 95% to quantitative.

Chromatogram of Protein Mixture on CM 1500 Column

Chromatogram of protein Mixture on CM 3000 Column