Weak Anion Exchanger: DEAE 1500 and DEAE 3000

DEAE 1500 is prepared from 10µm particles of highly cross-linked PS-DVB beads with pore diameter of 500 Å. Similarly, DEAE 3000 is prepared from 10 µm particles of PS-DVB beads with average pore diameter of larger than 1000Å. The packing has an optimum pore size and is suitable for loading and analysis of large globular proteins.

In coupling of the polymer chains of diethylaminoethyl (DEAE) groups with the hydrophilic surface of the rigid macroporous polymer matrix, weak anion separations can be achieved over the wide pH range of 3 to 9. Elution is accomplished with gradients of increasing ionic strength or pH.

The dynamic loading capacity for BSA is about 50 mg/ml of column volume. Recoveries of proteins are typically from 95% to quantitative.

Chromatogram for Protein Mixture on DEAE 1500 Column

Chromatogram for Protein Mixture on DEAE 3000 Column