SP 1500 is prepared from 10 µm particles of highly
cross-linked PS-DVB beads with pore size of 500 Å.
Similarly, SP 3000 is produced from 10 µm particles of
highly cross-linked PS-DVB beads with pore size of larger
than 1000 Å. After being coated with neutral,
hydrophilic layer, the polymer is chemically bonded with
the polymer chains of sulfopropyl (SP) groups to provide
the optimum density of strong cation exchange
functionalities for macromolecule separations.
The media
is compatible with aqueous solvents in the pH range of 1
to 14, as well as with most organic solvents. Elution of
proteins can be achieved at relatively low ionic strength
(< 0.5 M NaCl) in 15 minutes at a flow rate of 1.0
mL/min. Strong cation separations can be achieved over the wide pH range
of 3 to 12. Because of the hydrophilic nature of the
adsorbents, proteins with pI value close to or below the
mobile phase pH will not be retained on Hydrocell cation
exchange columns.
 Chromatogram
of Protein Mixture on SP 1500 Column
Chromatogram of Protein Mixture on SP
3000 Column
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