Strong Anion Exchanger: QA 1500 and QA 3000

QA 1500 and QA 3000 are quaternary amine anion exchanger designed for the rapid separation of proteins and enzymes. The rigid macroporous PS-DVB matrix with a hydrophilic surface has been bonded with the polymer chains of diethylmethylaminoethyl groups to provide the optimum density of strong anion exchange functionalities for macromolecule separations.

The media is compatible with aqueous solvents in the pH range of 1 to 14 and with most organic solvents. It provides a mild, nondenaturing adsorbent from which proteins can be eluted with buffers of moderate ionic strength (< 0.5M NaCl) in 15 minutes at a flow rate of 1.0 ml/minute. Strong anion separations can be achieved over the wide pH range of 3 to 11. HYDROCELL anion exchangers are very hydrophilic, proteins with pI close to or above the mobile phase pH will not be retained under these conditions.

Chromatogram of Protein Mixture on QA 1500 Column

Chromatogram of Protein Mixture on QA 3000 Column