Ion Exchange Columns

HYDROCELL ion exchange adsorbents were developed by BioChrom Labs, Inc. for proteins, peptides, oligonucleotides, DNA fragments and other biomolecule separations.

They are based on the coatings of the neutral hydrophilic layer on the rigid macroporous polystyrene-divinylbenzene (PS-DVB) matrix, followed by chemical immobolization of the polymer chains of bonded phase on the hydrophilic surface.

The packings do not have any weak ester or amide linkages as do the other polymeric gels. Their chemical and physical stabilities allow rapid equilibration and fast clean-up using high salt, HCl, NaOH or organic solvents.

Unlike some columns where hydrophobic retention can complicate ion exchange chromatography by producing irreversible adsorption, HYDROCELL ion exchangers are very hydrophilic. Elution of proteins can be achieved with buffers of moderate ionic strength, typically less than 0.5M NaCl, in 15 minutes at a flow rate of 1.0 ml / minute.

With a pH range of 1 to 14 and pressure limit of 4000 psi, HYDROCELL columns are ideal for the separation and analysis of a wide range of biological materials.