Hydrocell DEAE 3000

Protein Mixture

Mobile Phase:
     Eluent A: 10 mM Tris, pH 8.0
     Eluent B: Eluent A + 0.5 M NaCl, pH 8.0

Gradient: Linear 0-50% B in 20 min.

Detection: UV 280 nm

Flow Rate: 0.25 mL / min

Injection : 20 uL

Peak Identification:
     1. Conalbumin
     2. Ovalbumin
     3. Soybean Trypsin Inhibitor