Hydrocell CM 3000

Protein Mixture

Mobile Phase:
Eluent A: 20 mM Potassium Phosphate, pH 6.0
Eluent B: Eluent A + 1 M NaCl, pH 6.0

Gradient: Linear 0-50% B in 20 minutes

Detection: UV 280 nm

Flow Rate: 1 mL/min

Injection: 20 µL

Peak Identification:
1. Chymotrysinogen A
2. Cytochrome C
3. Lysozyme