Hydrocell CM 1500

Protein Mixture
Column: 50 x 4.6 mm

Mobile Phase:
Eluent A: 20 mM Potassium Phosphate, pH 6.0
Eluent B: Eluent A + 0.5 M NaCl, pH 6.0

Gradient: Linear 0-100% B in 20 minutes

Detection: UV 280 nm

Flow Rate: 1 mL/min

Injection: 20 µL

Peak Identification:
1. Trypsinogen
2. Chymotrysinogen A
3. Lysozyme