Hydrocell C4 3000

Protein Mixture

Mobile Phase:
Eluent A: 2.5 M Ammonium Sulfate in Eluent B
Eluent B: 0.1 M Potassium Phosphate (pH 7.0)

Gradient: Linear 0-100% B in 20 minutes

Detection: UV 280 nm

Flow Rate: 1.0 mL / min.

Peak Identification:
1. Ribonuclease A
2. Lysozyme
3. Chymotrysinogen A