Hydrophobic Interaction Columns

Hydrophobic Interaction Chromatography (HIC) separates proteins by mechanisms similar to Reversed-Phase Chromatography but under gentle reverse salt gradient elution conditions in aqueous buffers. Since no organic solvent is used, biological activity has a much higher probability of being retained.

HYDROCELL C3 1500, C4 1500 and Phenyl 1500 are prepared from 10 µm particles of hydrophilic PS-DVB beads with pore diameter of 500 Å. Similarly, C3 3000, C4 3000 and Phenyl 3000 are produced from 10 µm particles of hydrophilic, macroporous PS-DVB beads with average pore diameter of 1500Å.

The packing materials have been chemically bonded with allyl (C3), butyl (C4) or phenyl as a hydrophobic group. These packings are the same as HYDROCELL ion exchange packings which do not have any weak ester linkages as do the other commercial products. They are stable in a pH range of 1 to 14 and are compatible with most organic solvents. The columns can be operated at high flow rates for rapid equilibration and fast clean-up.

HYDROCELL HIC Columns are available in both stainless steel and PEEK. Please refer to Ordering Information for available column size and catalog order number information.

The dynamic protein loading capacity determined by Frontal Uptake procedures are about 30 mg, 30 mg and 35 mg of lysozyme per mL of column volume on C3 1500, C4 1500 and Phenyl 1500 respectively. The practical loading capacity is about 0.4 - 0.6 mg of protein on a 50 x 4.6mm column. For detailed chromatograms on each packing material, please click the following:

Chromatogram of Protein Mixture on C3 1500

Chromatogram of Protein Mixture on C3 3000

Chromatogram of Protein Mixture on C4 1500

Chromatogram of protein mixture on C4 3000

Chromatogram of Protein Mixture on Phenyl 1500

Chromatogram of Protein Mixture on Phenyl 3000

Please call 812-234-2558 for Trouble Shooting and additional technical support.